Document 3176
 DOCN  M94A3176
 TI    Characterization of enhancer-binding proteins that recognize HTLV-I LTR.
 DT    9412
 AU    Nyunoya H; Shimotohno K; National Cancer Center Research Institute,
       Tokyo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0165). Unique
       Identifier : AIDSLINE ICA10/94369399
 AB    OBJECTIVE: The promoter activity of HTLV-I can be controlled by tax-gene
       product as well as cyclic AMP or TPA. Factors related to the cellular
       signal-transduction pathways may be involved in the transcriptional
       control. To identify such factors, we have cloned cDNAs for multiple
       enhancer-binding proteins and characterized them. METHODS:
       Oligonucleotide probe containing the enhancer sequence was used for cDNA
       cloning. DNA-binding specificity and other properties were examined by
       using recombinant proteins made in E. coli. RESULTS: We have identified
       three Tax-responsive element-binding proteins (TAXREB), which all have a
       bZIP structure. TAXREB 67 and TAXREB302 recognized a core sequence
       (TGACG) of cyclic AMP responsive element; TAXREB107 recognized the
       downstream flanking sequence (TCCCCC). TAXREB67 was shown to be a
       phosphoprotein in vivo and to be phosphorylated by cdc-2 kinase in
       vitro. DISCUSSION AND CONCLUSIONS: The virus gene expression could be
       regulated by multiple transcription factors bound to the LTR. Responses
       to various extracellular stimuli may be different depending on
       cell-type. Systematic analyses of such factors should be required for
       further study.
 DE    Base Sequence  Cloning, Molecular  Comparative Study  Cyclic
       AMP/METABOLISM  DNA-Binding Proteins/*METABOLISM  DNA, Viral/*METABOLISM
       *Enhancer Elements (Genetics)  Escherichia coli  Gene Expression
       Regulation, Viral  HTLV-I/*GENETICS/METABOLISM  Oligonucleotide Probes
       Promoter Regions (Genetics)  Recombinant Proteins/METABOLISM
       *Repetitive Sequences, Nucleic Acid  Signal Transduction  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).